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91.
AIM To observe the effect of Fuzilizhong decoction on the inflammatory damage of non-alcoholic fatty liver disease (NAFLD) rats and to explore its mechanism. METHODS SPF male SD rats were randomly divided into 6 groups: control group, model group, high dose (20 mg·kg-1·d-1), middle dose (10 mg·kg-1·d-1), low dose (5 mg·kg-1·d-1) Fuzilizhong decoction group and Yishanfu (30 mg·kg-1·d-1)group, 8 rats in each group. A NAFLD rat modelwas established by intragastric administration of fat emulsion for 4 weeks. Then the drug was given for 4 weeks in each treatment group. HE staining was performed to observe the histopathological changes of the rat liver.The serum levels of interleukin-2(IL-2), IL-6 and tumor necrosis factor-α(TNF-α) were measured by ELISA. The expression of toll like receptor 4(TLR4) and NF-κB p65 in liver tissues at mRNA and protein levels was determined by RT-qPCR and Western bolt,respectively. RESULTS Compared with control group, the inflammatory damage of liver tissue was more serious, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression TLR4 and NF-κB p65 in liver tissues were significantly increased in model group(P <0.05). However, compared with model group, the liver pathological changes in each treatment group were significantly relieved, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression of TLR4 and NF-κB p65 in liver tissues were significantly reduced(P <0.05).In addition, the changes of TLR4 and p-NF-κB p65 protein levels in liver tissue were consistent with the changes of TLR4 and NF-κB p65 mRNA. CONCLUSION Fuzilizhong decoction attenuates the inflammatory damages of NAFLD in rats by inhibiting TLR4/NF-κB p65 signaling pathway. 相似文献
92.
Engineering designed mode has entered a new stage because of the development of network. New calculating system based on network appeared. By analyzing the common B/S architecture, this paper brings forward an reformative architecture based on Web services and ASP. NET in order to build the new mode of engineering calculation. It can fully implement the sharing of the resources and provide the engineering designers a wonderful calculating environment, and the working eifficiency of designers can be enhanced. 相似文献
93.
Pod shatter susceptibility was investigated in Brassica napus germplasm and shatter resistant species of B. juncea and Sinapis alba. The comparisons were made by measuring seed yield in field plots, detached pod rupture energy (RE) and the half‐life of pod‐opening. Pod shatter resistance was significantly greater in B. napus lines derived from interspecific hybridizations of B. napus with B. rapa, B. carinata and B. juncea, than common B. napus cultivars. While these lines exhibited no significant difference in resistance to pod shatter than B. juncea, an entry of S. alba had no yield loss caused by pod shatter. Resistance to pod shatter was characterized in the field as little or no yield loss after full maturity, delayed shattering in time, and stable yield performance under variable climatic conditions during pod maturity. Yield loss caused by pod shatter ranged from a low of 4% for the B. juncea cv. ‘AC Vulcan’ to a high of 61% for the black seeded B. napus line DH12075 in 2‐year field trials after 1 month maturity. Pod shatter resistance was not significantly associated with specific plant and pod morphological traits, except pod length (P = 0.005) in tested materials. Field visual scores of pod shatter through inspections of average pod shatter per plant within plots were highly correlated with plot yield loss. Indoor quantitative evaluations of pod strength using a pendulum machine to measure pod RE and random impact test to measure half‐life of pod‐opening resistance were highly correlated with field yield loss. Multiple evaluations of pod shatter in method and in time after pod maturity are recommended for reliable evaluation of pod shatter resistance. 相似文献
94.
The Effect of Chromosome 1B/1R Translocation on the Yield Potential of Certain Spring Wheats (Triticum aestivum L.) 总被引:2,自引:0,他引:2
Nearly 50 percent of the 1988 advanced breeding lines of the CIMMYT bread wheat breeding program possess the 1B/1R homozygous translocation. Hence, a trial was conducted to estimate the effect of 1B/1R chromosome translocation on the yield potential of some of our high-yielding spring wheats, where non-limiting levels of fertility, moisture, preventive pest and disease programs were used. In conclusing the 1B/1R lines seemed to have increased their above-ground biomass yield, number of spikes per meter2, 1000-grain weight and test weight. They also exhibited a slight advantage over the 1B homozygous lines on grain yield. The observed difference, however, was non-significant, as was the plant height difference observed among the two groups. Varietal comparisons indicated that the 1B/1R group headed later than the 1B group. 相似文献
95.
Chloroplast (cp) and mitochondrial (mt) DNA restriction profiles of a cytoplasmic male sterile (CMS) line of Brassica juncea and its maintainer line were compared and found to be markedly different. Comparison of cpDNA restriction profiles of fifty different species of genus Brassica and some allied genera showed that the cpDNA profiles of CMS lines were similar to that of B. tournefortii for twenty different restriction endonucleases. This CMS system is thus not of spontaneous origin as reported earlier, but is alloplasmic in nature. Comparison of restriction profiles of mtDNA of B. tournefortii and CMS lines revealed some differences which might either be due to changes in DNA pattern during the transfer, or, due to the cytoplasm coming from a B. tournefortii line different from the one used in this study. 相似文献
96.
97.
A. SRIVASTAVA A. MUKHOPADHYAY N. ARUMUGAM V. GUPTA J. K. VERMA D. PENTAL A. K. PRADHAN 《Plant Breeding》2004,123(2):204-206
The objective of this study was to broaden the genetic base in oleiferous Brassica juncea by resynthesis, using 10 diverse parental lines of oleiferous B. rapa and two lines of B. nigra of both Indian and exotic origin. Out of 14 crosses attempted using B. rapa as the female parent, eight were successful. Embryo rescue was necessary to obtain interspecific plants. A total of 29 fertile interspecific plants were obtained after colchicine treatment. In the S2 generation, the expression of component characters in the majority of the resynthesized plants showed a negative trend. The resynthesized B. juncea lines are being maintained through repeated selfing and selection at each generation for desirable plant types. This process will continue till the progeny lines of the desirable plants achieve uniformity. 相似文献
98.
99.
贮藏年限对油菜种子发芽特性的影响 总被引:10,自引:1,他引:10
采用普通发芽法对本所在昆明自然贮藏条件下贮存了1-17年的某蓝型油菜(B.napusL.)种子的发芽率,始萌发时间、萌发高峰期及幼苗素质等发芽特性的变化进行了研究,结果表明:甘蓝型油菜种子的发芽率随贮存年限的增加而呈阶梯式的下降,而且随着贮存年限的增加,种子始萌发所需时间及萌发高峰期出现的时间也随之增加,峰高降低,幼苗素质下降,根据测定结果,在昆明自然贮藏条件下,甘蓝型油菜种子的贮藏寿命为8-9年,安全贮藏期为1-7年,相关分析表明,贮藏1-7年的甘蓝型油菜种子的发芽率与贮存年限呈极显著负相关,相关系数r=-0。9527**,回归方程y-93.07-0.59x。 相似文献
100.